Malignant peripheral nerve sheath tumors are t(X;18)-negative sarcomas. Molecular analysis of 25 cases occurring in neurofibromatosis type 1 patients, using two different RT-PCR-based methods of detection.

摘要: To verify the absence of synovial sarcoma translocation t(X;18) (SYT-SSX) in malignant peripheral nerve sheath tumors, 34 tumor samples from 25 neurofibromatosis type 1 patients were examined two independent laboratories (Bordeaux, France, and Lausanne, Switzerland) using reverse transcriptase polymerase chain reaction (RT-PCR)-based techniques. RNA was extracted paraffin blocks standard methods, transcribed, conventional (in one laboratory) versus real-time other PCR performed. Twenty-seven 19 negative for both laboratories; six additional tumors that t(X;18)-negative laboratory gave noninterpretable results other, due to lack internal positive controls; case places. In conclusion, do not bear (SYT-SSX). Laboratories use PCR-based techniques diagnostic purposes would benefit quality assurance programs.