Isolation and culture of skin fibroblasts.
作者： Laure Rittié , Gary J. Fisher
DOI: 10.1385/1-59259-940-0:083
关键词:
摘要: Dermal fibroblasts synthesize and organize skin connective tissue (dermis). Fibroblast cultures provide a powerful tool for investigating both the molecular mechanisms that regulate production pathophysiology of fibrotic diseases. In this chapter, we describe detailed procedures establishing maintaining primary adult human fibroblasts. We also protocols constructing three-dimensional dermal equivalent culture models.
springer.com
springerlink.com参考文章(21)
M Aumailley, T Krieg, G Razaka, P K Müller, H Bricaud, Influence of cell density on collagen biosynthesis in fibroblast cultures Biochemical Journal. ,vol. 206, pp. 505- 510 ,(1982) , 10.1042/BJ2060505
Laure Rittié, Gary J Fisher, UV-light-induced signal cascades and skin aging Ageing Research Reviews. ,vol. 1, pp. 705- 720 ,(2002) , 10.1016/S1568-1637(02)00024-7
Roxane Pouliot, Lucie Germain, François A. Auger, Nathalie Tremblay, Julianna Juhasz, Physical characterization of the stratum corneum of an in vitro human skin equivalent produced by tissue engineering and its comparison with normal human skin by ATR-FTIR spectroscopy and thermal analysis (DSC) Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. ,vol. 1439, pp. 341- 352 ,(1999) , 10.1016/S1388-1981(99)00086-4
Cornelia Mauch, Bernhard Adelmann-Grill, Atsushi Hatamochi, Thomas Krieg, Collagenase gene expression in fibroblasts is regulated by a three-dimensional contact with collagen FEBS Letters. ,vol. 250, pp. 301- 305 ,(1989) , 10.1016/0014-5793(89)80743-4
Pamela D. Arora, Nazanin Narani, Christopher A.G. McCulloch, The compliance of collagen gels regulates transforming growth factor-β induction of α-smooth muscle actin in fibroblasts American Journal of Pathology. ,vol. 154, pp. 871- 882 ,(1999) , 10.1016/S0002-9440(10)65334-5
R. Roguet, C. Cohen, K.G. Dossou, A. Rougier, Episkin, a reconstituted human epidermis for assessing in vitro the irritancy of topically applied compounds. Toxicology in Vitro. ,vol. 8, pp. 283- 291 ,(1994) , 10.1016/0887-2333(94)90195-3
E. Bell, B. Ivarsson, C. Merrill, Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 76, pp. 1274- 1278 ,(1979) , 10.1073/PNAS.76.3.1274
B.J. NICKOLOFF, R.S. MITRA, J.T. ELDER, G.J. FISHER, J.J. VOORHEES, Decreased growth inhibition by recombinant gamma interferon is associated with increased transforming growth factor-α production in keratinocytes cultured from psoriatic lesions British Journal of Dermatology. ,vol. 121, pp. 161- 174 ,(1989) , 10.1111/J.1365-2133.1989.TB01795.X
E Bell, H. Ehrlich, D. Buttle, T Nakatsuji, Living tissue formed in vitro and accepted as skin-equivalent tissue of full thickness Science. ,vol. 211, pp. 1052- 1054 ,(1981) , 10.1126/SCIENCE.7008197
L. Hayflick, P.S. Moorhead, The serial cultivation of human diploid cell strains. Experimental Cell Research. ,vol. 25, pp. 585- 621 ,(1961) , 10.1016/0014-4827(61)90192-6