Carboxylesterases expressed in human colon tumor tissue and their role in CPT-11 hydrolysis.
作者： William F. Bosron , Daryl J. Murry , Sara K. Quinney , Sonal P. Sanghani , Wilhelmina I. Davis
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摘要: Purpose: The purpose is to develop new analytical methods study the expression profile of CPT-11 carboxylesterases and topoisomerase I in colon tumor samples understand impact their on metabolism chemotherapy. Experimental Design: We investigated 24 tumors for CES1A1 , CES2 CES3 hBr-3 genes by real-time PCR correlated gene with activity assays. relative abundance carboxylesterase isoenzymes was determined PCR. Activity assays performed extracts included hydrolase, 4-methylumbelliferyl acetate Additionally, nondenaturing gel electrophoresis staining showed distribution carboxylesterases. Results: detect human tumors. were unable (also called hCE-3 ) liver, colon, or brain. find large interindividual variation, ≥150-fold, both genes, 23-fold 66-fold I. Only ( P Conclusions: conclude that most abundant responsible hydrolysis. This pilot reinforces hypothesis there a variation may contribute therapeutic outcome and/or toxicity therapy cancer.
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