Purification and properties of the Ascaris pyruvate dehydrogenase complex.
作者： Richard Komuniecki , Patricia R. Komuniecki , Howard J. Saz
DOI: 10.1016/0005-2744(79)90219-5
关键词:
摘要: Abstract The pyruvate dehydrogenase complex (pyruvate:lipoate oxidoreductase (decarboxylating and acceptor-acetylating), EC 1.2.4.1) has been isolated from Ascaris muscle mitochondria purified to near homogeneity by differential centrifugation, (NH4)2SO4 fractionation calcium phosphate gel-cellulose chromatography. It is similar in shape, size physical characteristics complexes mammalian sources. an absolute dependence on CoA, NAD+ for activity competitively inhibited acetyl-CoA NADH. However, much higher NADH/NAD+ ratios are necessary inhibit activity, suggesting regulation the more reduced state of pyridine nucleotide pool mitochondria.
elsevier.com
sci-hub.se 参考文章(33)
Elliot Juni, Gloria A. Heym, ACYLOIN CONDENSATION REACTIONS OF PYRUVIC OXIDASE Journal of Biological Chemistry. ,vol. 218, pp. 365- 378 ,(1956) , 10.1016/S0021-9258(18)65900-9
Z S de Mata, H J Saz, D J Pasto, 2-methylacetoacetate reductase and possible propionyl coenzyme A condensing enzyme activity in branched chain volatile fatty acid synthesis by Ascaris lumbricoides. Journal of Biological Chemistry. ,vol. 252, pp. 4215- 4224 ,(1977) , 10.1016/S0021-9258(17)40253-5
Emil Kmetec, Ernest Bueding, Succinic and reduced diphosphopyridine nucleotide oxidase systems of Ascaris muscle. Journal of Biological Chemistry. ,vol. 236, pp. 584- 591 ,(1961) , 10.1016/S0021-9258(18)64408-4
John P. Blass, Carole A. Lewis, Kinetic properties of the partially purified pyruvate dehydrogenase complex of ox brain Biochemical Journal. ,vol. 131, pp. 31- 37 ,(1973) , 10.1042/BJ1310031
Vincent E. Price, Robert E. Greenfield, Liver catalase. II. Catalase fractions from normal and tumor-bearing rats. Journal of Biological Chemistry. ,vol. 209, pp. 363- 376 ,(1954) , 10.1016/S0021-9258(18)65563-2
Irving Seidman, Nathan Entner, Oxidative enzymes and their role in phosphorylation in sarcosomes of adult Ascaris lumbricoides. Journal of Biological Chemistry. ,vol. 236, pp. 915- 919 ,(1961) , 10.1016/S0021-9258(18)64330-3
Taro Hayakawa, Masahiro Hirashima, Satoshi Ide, Minoru Hamada, Keiichiro Okabe, Masahiko Koike, Mammalian α-Keto Acid Dehydrogenase Complexes I. ISOLATION, PURIFICATION, AND PROPERTIES OF PYRUVATE DEHYDROGENASE COMPLEX OF PIG HEART MUSCLE Journal of Biological Chemistry. ,vol. 241, pp. 4694- 4699 ,(1966) , 10.1016/S0021-9258(18)99702-4
Tracy C. Linn, John W. Pelley, Flora H. Pettit, Ferdinand Hucho, Douglas D. Randall, Lester J. Reed, α-Keto acid dehydrogenase complexes: XV. Purification and properties of the component enzymes of the pyruvate dehydrogenase complexes from bovine kidney and heart Archives of Biochemistry and Biophysics. ,vol. 148, pp. 327- 342 ,(1972) , 10.1016/0003-9861(72)90151-8
W.W. Westerfeld, A colorimetric determination of blood acetoin. Journal of Biological Chemistry. ,vol. 161, pp. 495- 502 ,(1945) , 10.1016/S0021-9258(17)41484-0
Peter Kohler, H. J. Saz, Demonstration and possible function of NADH:NAD+ transhydrogenase from ascaris muscle mitochondria. Journal of Biological Chemistry. ,vol. 251, pp. 2217- 2225 ,(1976) , 10.1016/S0021-9258(17)33575-5