Influenza Vaccine Prepared by Photodynamic Inactivation of Virus

摘要: Summary and Conclusions Influenza virus (10 8 EID 50 /ml, PR-8 strain of type A) in the presence 10 -5.5 M toluidine blue was photodynamically inactivated by visible light. Although all detectable infectivity destroyed, immunizing hemagglutinating antigens were preserved. The photosensitive influenza-dye complex is readily dissociable, since virus-dye mixtures passed through cation resins yielded dye-free that regained its photoresistance. infected allantoic fluid could be hemagglutinin not affected. However, upon removal certain constituents dialysis or erythrocyte purification virus, viral became blue. results with purified are similar to those found mature poliovirus particle, for here also, required infective moiety become (9). vaccines photodynamic action formalin compared antigenicity mice. With vaccine, immunologic response mice equal produced formalin-inactivated vaccine but somewhat less than injection live virus. Such a might prove advantageous use strains have particularly sensitive formalin.