Photodynamic Inactivation of Herpesvirus

摘要: Photodynamic inactivation has been known since Raab, at the beginning of 20th century, observed that acridine was harmless to paramecia in dark but lethal when organisms were exposed visible light (1). Three decades later viruses shown be photosensitive (2, 3). However, assay methods 1930s crude, and no quantitative results reported. In 1958, Yamamoto (4) reported first studies on photodynamic bacterial virus; 1960, Hiatt et al. (5) extended this work a number DNA-containing animal viruses, found RNA-containing enteroviruses resistant photosensitization. We have learned naturally photoresistant can made if virus is grown cells maintained with medium containing proflavine, neutral red, or orange. During replication virus, photoreactive dye becomes incorporated within structure (6–10). If one looks through literature, see most laboratories titers could reduced markedly by “dye-light” treatment, usually some persisted. The point that, as practiced, photoinactivation may not complete. Transformation such preparations which infectious still present (11) cannot said caused photoinactivated virus. procedures described papers from our laboratory are carefully followed, total (12–17).