Irreversible Photosensitization of Viruses.

摘要: Abstract The reaction between viruses and photoreactive dyes can result in a tightly bofnd, photosensitive, virus-dye complex. Herpesvirus was used as model to determine optimal photosensitizing conditions. These were incubation at pH 9 37° the presence of 10−4 M neutral red, proflavine, or toluidine blue absence visible light. free dye then removed from mixture by cationic resins, yield water-clear virus preparation which maintained its original titer. Subsequent exposure this light for few minutes rendered it noninfectiofs. Only herpesvirus measles totally photosensitive each above three dyes, using conditions described above. Reovirus influenza also completely photosensitized but parainfluenza, vaccinia, papova SV40 only blue. picornaviruses cofld not be under these Adenoviruses with red inactivated dark. Optimal temperatures fofnd necessary effective binding viruses. changes temperature reverse attachment render sensitized resistant If excess removed, all studied except photosenzitized