Meta-Analyses Qualify Metzincins and Related Genes as Acute Rejection Markers in Renal Transplant Patients

摘要: Definition of acute renal allograft rejection (AR) markers remains clinically relevant. Features T-cell-mediated AR are tubulointerstitial and vascular inflammation associated with excessive extracellular matrix (ECM) remodeling, regulated by metzincins, including metalloproteases (MMP). Our study focused on expression metzincins (METS), related genes (MARGS) in biopsies using four independent microarray data sets. own cases included normal histology (N, n = 20), borderline changes (BL, 4), (n 10) + IF/TA 7). MARGS enriched all sets were further examined mRNA and/or protein level additional patients. METS differentiated from BL, N a principal component analysis. Their correlated to Banff t- i-scores. Two classifiers, based (including MMP7, TIMP1), or established our validated the three Thirteen significantly patients comprising -9, TIMP1, -2, thrombospondin2 (THBS2) fibrillin1. RT-PCR microdissected glomeruli/tubuli confirmed -9 THBS2 results; immunohistochemistry showed augmentation MMP2, TIMP1 AR. patient serum. Therefore, differentially expressed especially MMP7/-9 represent potential molecular markers.