Complete development of hepatic stages of Plasmodium falciparum in vitro.

摘要: An in vitro model was developed to study the hepatic phase of Plasmodium falciparum, only malaria parasite lethal man. Primary cultures human hepatocytes were inoculated with sporozoites Brazilian and African strains P. falciparum. On days 1 through 7 after inoculation examination fluorescence-labeled Giemsa-stained preparations demonstrated presence many intracellular parasites. In three separate sets experiments all found be infected as 650 liver schizonts measuring up 40 micrometers. After addition red blood cells, intraerythrocytic forms falciparum detected on 12 13 by an immunofluorescence assay, indicating that cycle had been completed vitro.