Effects of sublethal doses of chlorfluazuron on the ovarian biochemical constituents of Spodoptera litura

摘要: This paper describes the effects of sublethal doses (LD 10 : 1.00 ng larva -1 ; LD 30 3.75 ) chlorfluazuron on amounts ovarian constituents during various stages development in common cutworm, Spodoptera litura (F.). Chlorfluazuron was applied topically to newly ecdysed fifth instars under laboratory conditions. In emerged adults, amount protein and treated larvae significantly reduced, but carbohydrate lipid were not affected compared with their respective controls. The order relative proportion DNA three developmental was: adults>newly pupae>newly larvae. Moreover, RNA larvae>newly adults> pupae. utricular greater (14.2 μgmg tissue) after first mating than second one spermatheca. However, reduced more it decreased rapidly, whereas steadily. On 5th day pupae, ecdysteroid titer 3.5 ± 0.8 pgmg . Initially, increased slowly then gradually, furthermore, sharply pupae before adult emergence. It peaked 1st emergence (52.0 1.5 thereafter. Therefore, is concluded that oogenesis S. These reductions an increase dose from are presumed be responsible for reduction fecundity caused by exposure chlorfluazuron. Key words: Chlorfluazuron, ecdysteroid, nucleic acids, oogenesis, litura, doses.