Muscle Transcriptional Profile Based on Muscle Fiber, Mitochondrial Respiratory Activity, and Metabolic Enzymes

摘要: Skeletal muscle is a highly metabolically active tissue that both stores and consumes energy. Important biological pathways affect energy metabolism metabolic fiber type in cells may be identified through transcriptomic profiling of the muscle, especially ante mortem. Here, gene expression was investigated malignant hyperthermia syndrome (MHS)-negative Duroc Pietrian (PiNN) pigs significantly differing for types slow-twitch-oxidative (STO) fast-twitch-oxidative (FTO) as well mitochondrial activity (succinate-dependent state 3 respiration rate). Longissimus samples were obtained 24 h before slaughter profiled using cDNA microarrays. Differential between PiNN associated with protein ubiquitination, stem cell pluripotency, amyloid processing, 3-phosphoinositide biosynthesis degradation pathways. In addition, weighted co-expression network analysis within breeds several modules proportion different types, respiratory activity, ATP metabolism. particular, results revealed strong correlations mitochondrion-associated STO (r = 0.78), fast-twitch glycolytic -0.98), complex I (r=0.72) COX 0.86). Other protein-kinase-activity enriched module positively correlated (r=0.93), while negatively FTO -0.72). contrast to PiNN, macromolecule catabolic process, actin cytoskeleton, transcription activator enzyme activities. Our highlight importance mitochondria oxidative capacity porcine breed-dependent molecular fibers.