Mobility, clustering, and transport of nerve growth factor in embryonal sensory cells and in a sympathetic neuronal cell line.

摘要: Abstract We have prepared a fluorescent conjugate of nerve growth factor (NGF) containing 8--10 rhodamine molecules attached to free carboxyl groups the protein. This analogue retained full binding capacity toward NGF receptors, antigenic properties, and potency stimulate differentiation embryonal chicken sensory ganglia cells in vitro. We used this study mobility distribution receptors on from dorsal root pheochromocytoma cell line (PC-12) that responds by differentiating along neuronal pathway. The (R-NGF) binds initially diffusely distributed mobile (D approximately 8 X 10(-10) cm2/sec) immature PC-12 cells. At 37 degrees C, receptor complexes cluster form immobile visible patches. These patches undergo endocytosis process consumes metabolic energy. Methylamine blocks formation remain dispersed at C. On differentiated cells, R-NGF aggregated sites. clusters are localized tip axon, main body. internalized axon transported retrogradely peripherey