Tissue Sources for Accurate Measurement of Germline DNA Genotypes in Prostate Cancer Patients Treated With Radical Prostatectomy.
作者： Nima C. Emami , Lancelote Leong , Eunice Wan , Erin L. Van Blarigan , Matthew R. Cooperberg
DOI: 10.1002/PROS.23283
关键词:
摘要: BACKGROUND Benign tissue from a tumor-containing organ is commonly the only available source for obtaining patient's unmutated genome use in cancer research. While it critical to identify histologically normal that independent of tumor lineage, few additional considerations are applied choice this material such measurements. METHODS Normal formalin-fixed, paraffin-embedded seminal vesicle, and urethral tissues, addition whole blood, were collected 31 prostate patients having undergone radical prostatectomy. Genotype concordance was evaluated DNA each relation blood. RESULTS Overall, there greater genotype call rate derived (97.0%) comparison with patient-matched vesicle tissues (95.9%, P = 0.0015). Furthermore, reference samples exhibited higher (94.1%) than (92.5%, P = 0.035). CONCLUSIONS These findings highlight heterogeneity between diverse sources measurement motivate consideration biases tumor-normal analyses. Prostate © 2016 Wiley Periodicals, Inc.
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