Bacteriophage lambda N-dependent transcription antitermination. Competition for an RNA site may regulate antitermination.

摘要: Bacteriophage lambda controls the expression of its early genes in a temporal manner by series transcription termination and antitermination events. This requires N protein as well host proteins called Nus, cis-acting sites nut. Following nut site, Nus bind to RNA modify complex termination-resistant form. The site is composite at least two components; one boxB hairpin structure which interacts with N. other boxA, nine-nucleotide sequence upstream boxB. To understand more about formation complex, we have characterized effect point mutations deletions boxA on antitermination. Point were found either enhance or reduce N-mediated Several deletions, hand, had little than eliminate requirement for NusB protein. explain these observations, propose that factors compete interact an inhibitor reaction. In addition, required prevent from binding boxA. results various nusB can be explained this model competition between RNA.