CLONING OF A THIRD MEMBER OF THE D52 GENE FAMILY INDICATES ALTERNATIVE CODING SEQUENCE USAGE IN D52-LIKE TRANSCRIPTS

摘要: Abstract D52 proteins are emerging as signalling molecules which may be regulators of cell proliferation. Having previously reported the existence human gene family, comprising hD52 and hD53 genes expressed in breast carcinoma, we report identification a novel hD54 ( TPD52L2 ), represents third family member. In situ mapping placed on chromosome 20q13.2–q13.3, localization distinct from those both genes. The identified cDNAs predicted three isoforms, suggesting that alternatively-spliced transcripts produced D52-like This was confirmed by directly sequencing reverse transcriptase–polymerase chain reaction (RT–PCR) products amplified developing adult rat tissues, performing sequence analyses tag divisions nucleotide databases. Alternative splicing sequences encoding two regions, termed ins2 ins3, one or more genes, with these alternative events being differentially regulated. functional consequences were examined characterizing protein–protein interactions mediated truncated isoform within yeast two-hybrid system. displayed altered interaction capabilities respect to full-length hD53, functions part alter encoded isoforms.