Characterization of a Leucine Zipper-containing Protein Identified by Retroviral Insertion in Avian Neuroretina Cells

摘要: We reported previously that post-mitotic chicken embryonic neuroretina (NR) cells are induced to proliferate following in vitro infection with RAV-1, a retrovirus does not carry an oncogene. NR cell multiplication results from the frequent activation and subsequent retroviral transduction of two related serine/threonine protein kinases, c-mil/c-raf or c-Rmil/B-raf genes. also showed very early event these proto-oncogenes is synthesis chimeric mRNAs containing viral cellular sequences joined by splicing mechanism. In current study, we have examined ability RAV-1 induce proliferation quail cells. By using reverse transcription-polymerase chain reaction technique, identified, several proliferating cultures infected mRNA splice donor site. These derived gene designated R10, which expressed through 1.9-kilobase (kb) detected tissues. A second transcript 2.3 kb specifically NR, where both transcripts developmentally regulated. The R10 cDNA encodes 251-amino acid polypeptide contains leucine zipper motif. It exhibits significant similarity putative D52/N8L protein, encoded be overexpressed human breast lung carcinomas. polyclonal antibodies specific for its amino-terminal zipper-containing regions, identified product as cytoplasmic 23 kDa cultured avian fibroblasts. 30 express 2.3-kb transcript. show, transcription/translation immunoprecipitation, can readily form homodimers, presumably