Effect of porcine somatotropin on in vivo glucose kinetics and lipogenesis in growing pigs.

摘要: Crossbred barrows were used for in vivo studies investigating hormonal regulation of lipogenesis. The first experiment examined an method determining rates Three infused with [U-14C]glucose and incorporation radioactivity into triglycerides was determined up to five biopsies subcutaneous adipose tissue obtained over 7 h. Incorporation linear after blood glucose specific had reached a plateau constant the entire infusion. For second experiment, eight pigs (71 +/- 2.5 kg) allocated one two treatments involving daily injections excipient (control) or porcine somatotropin (pST; 120 micrograms/kg BW). On d 10, beginning 15 h injection, lipid under both basal hyperinsulinemic/euglycemic conditions. Basal lipid, particularly fatty acids, markedly reduced (greater than 90%) during pST treatment. Although increased similar extent groups by hyperinsulinemia, pST-treated still exhibited lower rates. Based on kinetic data, decrease accretion primarily result rate de novo synthesis. Furthermore, reductions irreversible loss rate, feed intake that occur treatment quantitatively similar.