Differential transcriptional activation by v-myb and c-myb in animal cells and Saccharomyces cerevisiae.

摘要: The v-myb oncogene and its cellular homolog c-myb encode sequence-specific DNA-binding proteins which regulate transcription from promoters containing Myb-binding sites in animal cells. We have developed a Saccharomyces cerevisiae system to assay transcriptional activation by v-Myb c-Myb. In yeast strains integrated reporter genes, was strictly dependent upon both the Myb domain recognition element. BAS1, an endogenous Myb-related protein, not required for transactivation itself had no detectable effect on gene. Deletion analyses demonstrated that of C terminal previously mapped cells but S. cerevisiae. same is also efficient transformation myeloid v-Myb. contrast results cells, full-length c-Myb much stronger transactivator than protein bearing oncogenic N- C-terminal truncations These imply negative regulation own termini requires additional cell or small molecule present