Cellular and humoral immune reconstitution after autologous peripheral blood stem cell transplantation (PBSCT)

摘要: Immune reconstitution after autologous peripheral blood stem cell transplantation (PBSCT) is of particular interest because its importance for clinical outcome. Despite prolonged immunosuppression, especially CD4(+) cells, few infections neutrophil recovery occur. Only reactivation varicella zoster virus (VZV) more frequent in the first year transplantation. From August 1997 to May 2001, we prospectively evaluated 38 patients prior conditioning and during follow-up 12 months post-transplant antibodies [measles, mumps, rubella, polio, herpes simplex, zoster, mononucleosis, cytomegalovirus (CMV)] lymphocyte subpopulations by flow cytometry. CD3(+) T lymphocytes, CD8(+) B-lymphocyte our study confirms previous reports. Complete was not achieved leading a suppressed CD4/CD8 ratio. IgG antibody titers against measles, polio were present almost all pretransplant post-transplant, indicating persistent humoral immunity. counts with VZV ( n=5) significantly higher (median: 1201/microl 938/microl, respectively) than without 594/microl 482/microl, 6-12 post-transplant. Positive CMV n=19) also correlated 3-6 1050/microl 1056/microl, compared CMV-negative (738/microl 584/microl, respectively), although none suffered from disease. Therefore, conclude that viral can contribute T-cell PBSCT oligoclonal expansion antigen-specific memory cells.