Acetylene as a suicide substrate and active site probe for methane monooxygenase from Methylococcus capsulatus (Bath)
作者： S.D. Prior , H. Dalton
DOI: 10.1111/J.1574-6968.1985.TB00843.X
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摘要: Acetylene was shown to be an inhibitor of cell-free methane monooxygenase (MMO) activity in Methylococcus capsulatus (Bath). Inhibition demonstrated for both the soluble and particulate forms enzyme dependent on presence NADH oxygen. Inactivation complex irreversible due binding acetylene specific proteins complex. The use radiolabelled [14C]acetylene provided a method visualisation bound inhibitor: protein sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE). bind which are associated with methane-oxidising it is proposed that acts as suicide substrate.
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sci-hub.se 参考文章(19)
M P Woodland, H Dalton, Purification and characterization of component A of the methane monooxygenase from Methylococcus capsulatus (Bath). Journal of Biological Chemistry. ,vol. 259, pp. 53- 59 ,(1984) , 10.1016/S0021-9258(17)43620-9
J Colby, D I Stirling, H Dalton, The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds. Biochemical Journal. ,vol. 165, pp. 395- 402 ,(1977) , 10.1042/BJ1650395
J A De Bont, E G Mulder, Invalidity of the acetylene reduction assay in alkane-utilizing, nitrogen-fixing bacteria. Applied and Environmental Microbiology. ,vol. 31, pp. 640- 647 ,(1976) , 10.1128/AEM.31.5.640-647.1976
David I. STIRLING, Howard DALTON, Properties of the Methane Mono-oxygenase from Extracts of Methylosinus trichosporium OB3b and Evidence for Its Similarity to the Enzyme from Methylococcus capsulatus (Bath) FEBS Journal. ,vol. 96, pp. 205- 212 ,(1979) , 10.1111/J.1432-1033.1979.TB13030.X
John LUND, Marc P. WOODLAND, Howard DALTON, Electron transfer reactions in the soluble methane monooxygenase of Methylococcus capsulatus (Bath). FEBS Journal. ,vol. 147, pp. 297- 305 ,(1985) , 10.1111/J.1432-1033.1985.TB08750.X
David I. Stirling, Howard Dalton, Effect of metal-binding agents and other compounds on methane oxidation by two strains of Methylococcus capsulatus Archives of Microbiology. ,vol. 114, pp. 71- 76 ,(1977) , 10.1007/BF00429633
Christopher Walsh, Suicide substrates: mechanism-based enzyme inactivators Tetrahedron. ,vol. 38, pp. 871- 909 ,(1982) , 10.1016/0040-4020(82)85068-0
Howard Dalton, Roger Whittenbury, The acetylene reduction technique as an assay for nitrogenase activity in the methane oxidizing bacterium Methylococcus capsulatus strain bath Archives of Microbiology. ,vol. 109, pp. 147- 151 ,(1976) , 10.1007/BF00425127
Russell K. Hynes, Roger Knowles, Inhibition by acetylene of ammonia oxidation in Nitrosomonas europaea Fems Microbiology Letters. ,vol. 4, pp. 319- 321 ,(1978) , 10.1111/J.1574-6968.1978.TB02889.X
S. H. Stanley, S. D. Prior, D. J. Leak, H. Dalton, Copper stress underlies the fundamental change in intracellular location of methane mono-oxygenase in methane-oxidizing organisms: Studies in batch and continuous cultures Biotechnology Letters. ,vol. 5, pp. 487- 492 ,(1983) , 10.1007/BF00132233