Automated analysis of postoperative behaviour: assessment of HomeCageScan as a novel method to rapidly identify pain and analgesic effects in mice.

摘要: This study evaluated whether the automated behaviour recognition software 'HomeCageScan' (HCS) could detect changes and any positive analgesic effects in two mouse strains undergoing vasectomy (C3H/HeNCrl C57BL/6). Another objective was to test effectiveness of HCS differentiating between each treatment relative conventional manual analysis. Each control (unoperated) group consisted four mice strain. They were either untreated mice, given meloxicam alone (10 mg/kg) or saline 30 min prior isoflurane anaesthesia. The vasectomized received at 5, 10 20 mg/kg, again, Filming began one hour following surgery. filmed for 6 analysis then a further with HCS. In time-matched test, produced activity data that generated identical conclusions regarding strain differences. Both found C57BL/6 controls overall more active, but not vasectomy, when both types detected markedly reduced activity. Low-dose (5 had effect on postoperative mobility C3H/HeNCrl mice; however, increasing dose rate progressively this. These also Overall, provided sufficiently accurate rapid method analysing encouraging prolonged assessments future. capability possibility training recognize greater range behaviours, including pain-specific indicators, should be considerable value assessing rats. would allow requirements investigated rodent models than is currently feasible methods.