Exosomal onco-miRs from serum of patients with adenocarcinoma of the esophagus: comparison of miRNA profiles of exosomes and matching tumor

摘要: Diagnostic markers are needed for achieving a cure in esophageal cancer, detecting tumor cells earlier. Exosomes bioactive vesicles secreted by into surrounding body fluids. Exosome formation, cargo content, and delivery have major impact cancer development. This is the first isolation of exosomes from serum patients with adenocarcinoma esophagus comparison exosomal miRNA profiles matching primary normal tissues. RNA was extracted profiling real-time TaqMan miR arrays. The cargo, tumor, tissue subgroup been compared. “Exosomal onco-miRs” such as miR-223-5p, miR-223-3p, miR-483-5p, miR-409-3p, miR-196b-5p, miR-192-5p, miR-146a-5p, miR-126-5p identified part being overexpressed corresponding compared to normal. Upregulation miR-223-5p miR-483-5p (p = 0.034, p = 0.017) has verified an independent cohort 43 T2-3 adeno- squamous cell carcinoma. In contrast, miR-224-5p, miR-452-5p, miR-23b-5p, miR-203-5p, miR-1201-5p, miR-149-5p, miR-671-3p, miR-944-5p, miR-27b-3p, miR-22-3p be significantly downregulated versus merely or not detectable exosomes. novel, stable, noninvasive source diagnosis therapy monitoring cancer. Oncogenic shuttle miRNAs present may contribute understanding how spread their oncogenic potential environment. “exosomal seem play role applied esophagus.