Nicotine Metabolism in Liver Microsomes from Rats with Acute Hepatitis or Cirrhosis
作者： Yoshihiko Funae , Yukio Kuroiwa , Takemi Yoshida , Toshinori Yamamoto , Kanae Iwata
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摘要: Nicotine exerts a number of physiological effects. is absorbed through the lungs with smoking and rapidly metabolized in humans. Although it mainly liver, effects liver injuries on nicotine metabolism are not clear. The purpose this study was to clarify metabolism. Rats were treated D-galactosamine (GalN) or thioacetamide (TA), induce acute hepatitis cirrhosis, respectively. Serum transaminase levels significantly elevated model rats both types injury. Cytochrome P450 (CYP) cytochrome b 5 contents microsomes decreased TA-treated cirrhotic but GalN-treated hepatitic rats. major metabolic pathways nicotine, i.e. cotinine formation catalyzed by CYP nicotine-1′- N -oxide flavin-containing monooxygenase, investigated these rat microsomes. Formation from changed rats, comparison controls, By immunoblotting, decreases CYP1A2, CYP2B2, CYP2C, CYP2E1 protein recognized It also shown that maximal velocity values for decreased, compared controls. These results suggested reduction cirrhosis due monooxygenase expression levels.
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