Transgenic expression of the TRI101 or PDR5 gene increases resistance of tobacco to the phytotoxic effects of the trichothecene 4,15-diacetoxyscirpenol.

摘要: Mycotoxins are fungal secondary compounds that toxic to vertebrates. Their presence in food and feeds, as the result of disease crops, can present a danger animal or human health. Many mycotoxins have also been shown be phytotoxic some cases, such with trichothecenes produced by wheat head blight fungus Fusarium graminearum, may act virulence factors. Antibiotic-producing organisms, including fungi, protect themselves from their own toxins metabolic alteration compound, modification target site action exporting compound extracellular space. We tested effectiveness adapting two these strategies, transport, plant cells deleterious effects trichothecene 4,15-diacetoxyscirpenol (DAS). Tobacco plants were transformed either Saccharomyces cerevisiae gene PDR5, which encodes multi-drug transporter, sporotrichioides TRI101, 3-O-acetyltransferase. Both genes conferred significant increased tolerance DAS measured sensitive seed germination assay. Expression PDR5 TRI101 seed-specific manner crop could lower incidence well reduce mycotoxin levels within seed.