Agrobacterium inoculation techniques for plant tissues

作者: Nancy L. Mathis , Maud A. W. Hinchee

DOI: 10.1007/978-94-011-0511-8_8

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摘要: Agrobacterium-mcdiatcd transformation of plants has been adapted to many dicotyledonous plant species. The wide applicability this method is a result several factors: the broad host range Agrobacterium [2, 6], regeneration responsiveness different expiant tissues 5], and utility selectable marker genes [1]. Tobacco (Nicotiana tobacum) easily transformed using Agrobacterium, basic procedures used with tobacco are similar those for other species 3]. For reason, an excellent illustration steps in Agrobacterium-mediated transformation. Although techniques transforming can be directly applied species, it sometimes necessary modify procedure successful

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