A single amino acid interchange yields reciprocal CTL specificities for HIV-1 gp160
作者： H Takahashi , S Merli , S. Putney , R Houghten , B Moss
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摘要: For the IIIB isolate of human immunodeficiency virus type-1 (HIV-1), immunodominant determinant envelope protein gp160 for cytotoxic T lymphocytes (CTLs) H-2d mice is in a region high sequence variability among HIV-1 isolates. The general requirements CTL recognition peptide antigens and relation to natural variation HIV were investigated. this purpose, line specific homologous segment from MN restricted by same class I major histocompatibility (MHC) molecule (Dd) as IIIB-specific CTLs was raised immunized with MN-env-recombinant vaccinia virus. MN-specific completely non-cross-reactive. Reciprocal exchange single amino acid between two sequences, which differed 6 15 residues, led complete reversal specificity peptides sensitizing targets, such that lysed targets exposed singly substituted vice versa. These data indicate importance residues defining epitopic have implications both effect immune pressure on selection viral mutants design effective vaccines.
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