Evolutionarily Conserved Alternative Splicing Across Monocots

作者: Wenbin Mei , Lucas Boatwright , Guanqiao Feng , James C. Schnable , W. Brad Barbazuk

DOI: 10.1101/120469

关键词:

摘要: One difficulty when identifying and analyzing alternative splicing (AS) events in plants is distinguishing functional AS from noise. way to add confidence the validity of a splice isoform observe that it conserved across evolutionarily related species. We use high throughput method identify junction based RNA-Seq data nine plant species including: five grass monocots (maize, sorghum, rice, Brachpodium foxtail millet), plus two non-grass (bananan African oil palm), eudicot Arabidopsis basal angiosperm Amborella. In total, 9,804 within 19,235 genes were identified between 2 or more studied. grasses containing large regions synteny, frequency twice observed for outside synteny blocks. plant-specific RS RS2Z subfamilies, we both conservation divergence after whole genome duplication maize. addition, subfamilies are highly connected with R2R3-MYB networks. Furthermore, discovered network on harboring enriched phosphatases, kinases ubiquitylation genes, which suggests may participate regulating signaling pathways. These lay foundation studying monocots, particularly species, this resource identifies an additional layer genotype phenotype impact future crop improvement efforts.

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