Adaptation of Spodoptera exigua (Lepidoptera: Noctuidae) to barley trypsin inhibitor BTI-CMe expressed in transgenic tobacco.
作者： Pilar Lara , Felix Ortego , Elena Gonzalez-Hidalgo , Pedro Castañera , Pilar Carbonero
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摘要: Nicotiana tabacum plants were transformed with the cDNA of barley trypsin inhibitor BTI-CMe under control 35S CaMV promoter. Although transgene was expressed and protein active in homozygous lines selected, growth Spodoptera exigua (Lepidoptera: Noctuidae) larvae reared on transgenic not affected. The protease activity larval midgut extracts after 2 days feeding tobacco leaves from highest expressing plant showed a reduction 25% trypsin-like compared to that insects fed non-transformed controls. susceptibility digestive serine-proteases inhibition by confirmed staining gels. This decrease compensated significant induction leucine aminopeptidase-like carboxipeptidase A-like activities, while chymotrypsin-, elastase-, B-like proteases
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