On physico-chemical interactions between daunomycin and nucleic acids

摘要: Abstract 1. Daunomycin is a new antibiotic with cytotoxic and antimitotic activity. 2. When cells are incubated in the presence of daunomycin appears to be bound deoxynucleoproteins, or more precisely, deoxyribonucleic acid. 3. If native heat denatured acid from various sources added solution daunomycin, shows some physico-chemical changes, such as: (a) changes visible absorption spectrum, (b) ultraviolet (c) fluorescent emission, (d) polarographic behaviour. 4. digested by deoxyribonuclease, apurinic acid, pyrimidine derivatives, (nucleotides, nucleosides free bases) solution, no take place; on contrary spectrum caused purine derivatives only at very high doses. However, these appear different those produced DNA. 5. Ribonucleic also bind but indicated not exactly same as induced 6. DNA, nucleic certain sedimentation coefficient, viscosity, thermal denaturation-renaturation behaviour, optical 7. The nature bonds between acids discussed consideration given biological consequences this bond formation.