Differential cytotoxic properties of Helleborus niger L. on tumour and immunocompetent cells.

摘要: Abstract Ethnopharmacological relevance In Romanian folk medicine, Helleborus niger L. is used for the treatment of rheumatoid arthritis or viral infections and in complementary therapy, especially anthroposophic medicine (AM), where plant administered as an adjuvant to treat malignant diseases. present study, we investigated differential cytotoxic effects H. on human tumour healthy cells immune system vitro . Material methods Protoanemonin saponins, significant constituents extracts, were quantified five individual batches using validated HPLC methods. Further, impact proliferation capacity (MTT assay) well apoptosis necrosis induction a panel cell lines lymphocytes (combined annexin V propidium iodide staining) was monitored. addition, NK function (degranulation-CD107a assay IFN-gamma secretion) also since these immunocompetent are important control malignancies within body. Results Extracts induced inhibition not only lymphoblastic leukaemia (MOLT4; IC 50 : 171 µg/mL) but myosarcoma (SK-UT-1b; 304 µg/mL) melanoma (HT-144; 569 µg/mL) due apoptosis. Purified T significantly affected through presence high concentrations while bulk affected. cells’ anti-tumour functions expressed by degranulation IFN-y production unaffected extract. Since protoanemonin saponins have been reported literature exert effects, their content determined. Conclusions extracts exhibit cytotoxicity towards T- NK-cells.