Incorporation of rosuvastatin-loaded chitosan/chondroitin sulfate nanoparticles into a thermosensitive hydrogel for bone tissue engineering: preparation, characterization, and cellular behavior.
作者： Mahboubeh Rezazadeh , Maryam Parandeh , Vajihe Akbari , Zahra Ebrahimi , Azade Taheri
DOI: 10.1080/10837450.2018.1484765
关键词:
摘要: Rosuvastatin (RSV) has been shown to have significant impact on the simulation of bone regeneration after local injection. The current study aimed develop a localized controlled delivery system from RSV by incorporating RSV-loaded chitosan/chondroitin sulfate (CTS/CS) nanoparticles into thermosensitive Pluronic F127/hyaluronic acid (PF127/HA) hydrogel. CTS/CS were prepared ionic gelation, and various formulation variables was assessed using Box-Behnken design. Consequently, optimized incorporated PF127/HA Rheological properties, degradation rates hydrogels, release rate hydrogel examined. Mean particle size, zeta potential, entrapment efficiency, mean time confirmed as 283.2 ± 16 nm, -31.2 ± 6.8 mV, 63.1 ± 4.2%, 6.14 ± 0.3 h, respectively. containing 3% w/v existed solution with low viscosity at room temperature converted semisolid upon increasing 35 °C. Hydrogel engrafted showed during 48 h superior in vitro gel stability. As revealed cytotoxicity mineralization assays, incorporation particles led improvement osteoblast viability proliferation.
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