Search for poly(A) polymerase targets in E. coli reveals its implication in surveillance of Glu tRNA processing and degradation of stable RNAs.

摘要: Polyadenylation is a universal post-transcriptional modification involved in degradation and quality control of bacterial RNAs. In Escherichia coli, it admitted that any accessible RNA 3' end can be tagged by poly(A) tail for decay. However, we do not have yet an overall view the population polyadenylated molecules. The sampling RNAs presented here demonstrates rRNA fragments tRNA precursors originating from internal spacer regions rrn operons, particular, rrnB are abundant polymerase targets. Focused analysis showed Glu rrnG transcripts exhibit long trailers primarily removed PNPase to lesser extent RNase II polymerase. Moreover, trimming exoribonucleases precedes 5' maturation P. Interestingly, characterization accumulate deficient strain still harbouring leader degraded pathway involving This surveillance described defective also applies wild-type tRNA.