Preservation of immune response after laparoscopy.

摘要: We evaluated the immunologic responses following laparoscopic and open surgery by comparing delayed type hypersensitivity induration size before after each method of accessing abdominal cavity. One hundred thirty-two male Sprague-Dawley rats were sensitized with keyhole limpet hemocyanine (KLH). Animals challenged KLH phytohemaglutanin (PHA) 10 days sensitization. On day 14 initial sensitization animals randomly divided into three groups. Group one served as controls had no procedure performed, group two underwent peritoneal insufflation carbon dioxide gas to a pressure 6–8 mm Hg for half hour, in midline laparotomy which was closed hour. Each rat immediately at post-operatively. The area response challenges measured calipers 24 48 hours challenge. Results this skin testing showed that laparotomy, despite having normal preoperatively, significantly diminished both PHA when postoperatively. insufflated differences from control any time point examined. conclude DTH model is better preserved laparoscopy than laparotomy.