Kinetics of subtilisin and thiolsubtilisin.

作者: M. Philipp , M.L. Bender

DOI: 10.1007/BF00215583

关键词:

摘要: Subtilisin is a bacterial serine protease with broad specificity in the S1 subsite. It has been very extensively studied using variety of kinetic and physical techniques. A chemical derivative, thiolsubtilisin, subjected to similar studies order analyze effects OH SH conversion on enzyme activity.

参考文章(149)
Yuji KARASAKI, Motonori OHNO, Kinetic specificities of BPN' and Carlsberg subtilisins. Mapping the aromatic binding site. Journal of Biochemistry. ,vol. 84, pp. 531- 538 ,(1978) , 10.1093/OXFORDJOURNALS.JBCHEM.A132157
CH Johnson, JR Knowles, The binding of inhibitors to α-chymotrypsin Biochemical Journal. ,vol. 101, pp. 56- 62 ,(1966) , 10.1042/BJ1010056
M. OTTESEN, J.T. JOHANSEN, I. SVENDSEN, Subtilisin: Stability Properties and Secondary Binding Sites Structure–Function Relationships of Proteolytic Enzymes#R##N#Proceedings of the International Symposium, Copenhagen, June 16–18, 1969, No. 37 in the Series of the International Union of Biochemistry Sponsored Symposia. pp. 175- 187 ,(1970) , 10.1016/B978-0-12-211850-0.50019-7
M. Laskowski, I. Kato, T. R. Leary, J. Schrode, R. W. Sealock, Evolution of Specificity of Protein Proteinase Inhibitors Springer, Berlin, Heidelberg. pp. 597- 611 ,(1974) , 10.1007/978-3-642-87966-1_66
D.A. Matthews, R.A. Alden, J.J. Birktoft, T. Freer, J. Kraut, Re-examination of the charge relay system in subtilisin comparison with other serine proteases. Journal of Biological Chemistry. ,vol. 252, pp. 8875- 8883 ,(1977) , 10.1016/S0021-9258(17)38322-9
Francis S. Markland, Emil L. Smith, 16 Subtilisins: Primary Structure, Chemical and Physical Properties The Enzymes. ,vol. 3, pp. 561- 608 ,(1971) , 10.1016/S1874-6047(08)60407-2
K E Neet, A Nanci, D E Koshland, Properties of Thiol-Subtilisin THE CONSEQUENCES OF CONVERTING THE ACTIVE SERINE RESIDUE TO CYSTEINE IN A SERINE PROTEASE Journal of Biological Chemistry. ,vol. 243, pp. 6392- 6401 ,(1968) , 10.1016/S0021-9258(18)93152-2
Kazuyuki Morihara, Tatsushi Oka, Hiroshige Tsuzuki, Comparative study of various serine alkaline proteinases from microorganisms Archives of Biochemistry and Biophysics. ,vol. 165, pp. 72- 79 ,(1974) , 10.1016/0003-9861(74)90143-X