DNA Targeting Sequence Improves Magnetic Nanoparticle-Based Plasmid DNA Transfection Efficiency in Model Neurons

摘要: Efficient non-viral plasmid DNA transfection of most stem cells, progenitor cells and primary cell lines currently presents an obstacle for many applications within gene therapy research. From a standpoint efficiency viability, magnetic nanoparticle-based is promising vectoring technique because it has demonstrated rapid improved outcomes when compared to alternative methods. Recently, our research group introduced oscillating magnet arrays that resulted in further improvements this novel (pDNA) technology. Continued nanomagnetic techniques have focused primarily on nanoparticle (MNP) functionalization parameter optimization: confluence, growth media, serum starvation, oscillation parameters, etc. Noting none these parameters can assist the nuclear translocation delivered pDNA following MNP-pDNA complex dissociation cell’s cytoplasm, inclusion cassette feature theoretically justified. In study incorporation targeting sequence (DTS) transfecting model neurons, presumably from increased translocation. This observation became apparent comparing response dividing SH-SY5Y precursor non-dividing differentiated neuroblastoma cells.