[32] Microradioenzymic assays for the measurement of catecholamines and serotonin

摘要: Publisher Summary The chapter discusses the microradioenzymic assays for measurement of catecholamines and serotonin. Microenzymatic allow picogram levels biogenic amines in animal tissue. When these are coupled with microdissection techniques, monoamine metabolism can be studied sites as small individual brain nuclei. sensitivity assay allows determination after most areas. serotonin is based on procedure described initially by Saavedra et al . discussed Beavan Philips, major difference being that N-acetyltransferase obtained from Drosophila melanogaster used lieu a similar enzyme purified rat liver. In original radioenzymic serotonin, (NAT) advantages N-acetyltransferase. presents higher activity greater purity stability NAT undoubtedly increases reduces interassay variance 5-HT assay. Radioenzymic exploit, vitro , metabolic fates incorporating tritium ( 3 H) into structure O-methylated derivatives.