Protection against murine neonatal herpes simplex virus infection by lymphokine-treated human leukocytes.

摘要: One-week-old mice were protected against a uniformly lethal herpes simplex virus (HSV) infection by IL-2 alone, but especially the addition of human mononuclear cells (MC) plus IL-2. The dose response was biphasic. MC from cord blood did not enhance IL-2-mediated survival. Because effect or MC, ablated anti-IFN-gamma and neonates have an IFN-gamma production defect, protective (HuIFN-gamma) tested. adults cultured for 5 days in HuIFN-gamma afforded protection. At least 1 x 10(6) HuIFN-gamma-treated required with increasing survival to 10(7) MC. effector cell activity adherence, silica, L-leucine methyl ester treatment Leu-M3 C (all macrophage markers), OKT4 (CD4 marker). Use Leu-11, Leu-7, OKT3, OKT8 inhibit protection excluded NK T participation. In survival, ability produce anti-HSV antibody reconstituted. For first time after HuIFN vitro. We identified IFN-gamma-driven system murine neonatal HSV mediated adult- blood-derived CD4-positive macrophages. Protection is associated enhanced function reconstitution defect.