Induction of experimental allergic encephalomyelitis in PL/J and (SJL/J x PL/J)F1 mice by myelin basic protein and its peptides: localization of a second encephalitogenic determinant.

摘要: SJL/J, PL/J, and (SJL/J x PL/J)F1 mice were immunized with bovine, guinea pig, mouse, or rat myelin basic proteins (MBP) in adjuvant containing Mycobacterium tuberculosis H37Ra. Twenty-four 72 hr later, Bordetella pertussis vaccine was given i.v. All MBP tested induced experimental allergic encephalomyelitis (EAE) SJL/J F1 mice; however, bovine inactive PL/J mice. Each strain a similar manner peptic peptides, residues 1-37, 43-88, 89-169 of pig MBP. In contrast to the strain, which has been shown recognize major encephalitogenic determinant peptide 89-169, responded primarily an within 1-37. Analysis antibody levels showed that made no although anti-peptide antibodies consistently found. Conversely, well but only occasional animal significant response 89-169. more susceptible EAE than either parental as by percentage animals showing neurologic signs clinical severity.