5-(Pyren-1-yl)uracil as a base-discriminating fluorescent nucleobase in pyrrolidinyl peptide nucleic acids.
作者： Chalothorn Boonlua , Chotima Vilaivan , Hans-Achim Wagenknecht , Tirayut Vilaivan
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摘要: A pyrene-labeled uridine (U(Py)) monomer for a pyrrolidinyl peptide nucleic acid with an alternating proline/2-aminocyclopentanecarboxylic backbone (acpcPNA) was synthesized and incorporated into the PNA. The U(Py) base in acpcPNA could specifically recognize its complementary DNA strand as determined by thermal denaturation (T(m)) experiments. fluorescence of U(Py)-containing single-stranded very weak aqueous buffer. In presence target, enhanced significantly (2.7-41.9 folds, depending on sequences). enhancement specific to pairing between dA, making U(Py)-modified useful hybridization-responsive probe DNA-sequence determination.
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