Structure and expression of the rat apolipoprotein E gene.

摘要: The rat apolipoprotein E gene was isolated from a genomic library by screening with cDNA probe. nucleotide sequence of the plus 5' flanking region 623 nucleotides and 3' 234 determined. contained three introns, which were located in nontranslated regions coding for signal peptide mature protein. A "TATA box" TATAATT found beginning at -32. Two copies sequences GGGCGG or CCGCCC, potential binding sites transcription protein factor Sp 1, adjacent to TATA box, -52 -72. proximal region, up about 140 nucleotides, be highly conserved (82% homology) human. This GC-rich (68% G + C) self-complementary form hairpin loop structures, suggesting regulatory role this transcription. open reading frames promoter found, one first other second intron. Expression mRNA murine L cells transfected cloned detected levels comparable that liver. Rat expressed secreted into medium.