Antioxidant Activity/Capacity Measurement. 2. Hydrogen Atom Transfer (HAT)-Based, Mixed-Mode (Electron Transfer (ET)/HAT), and Lipid Peroxidation Assays
作者： Reşat Apak , Mustafa Özyürek , Kubilay Güçlü , Esra Çapanoğlu
关键词:
摘要: Measuring the antioxidant activity/capacity levels of food extracts and biological fluids is useful for determining nutritional value foodstuffs diagnosis, treatment, follow-up numerous oxidative stress-related diseases. Biologically, antioxidants play their health-beneficial roles via transferring a hydrogen (H) atom or an electron (e(-)) to reactive species, thereby deactivating them. Antioxidant activity assays imitate this action; that is, are measured by H transfer (HAT) e(-) (ET) probe molecules. can be monitored wide variety with different mechanisms, including HAT, ET, mixed-mode (ET/HAT) assays, generally without distinct boundaries between Understanding principal advantages, disadvantages measurement important proper selection method valid evaluation properties in desired applications. This work provides general up-to-date overview HAT-based, (ET/HAT), lipid peroxidation available measuring chemistry behind them, critical advantages drawbacks.
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