Extension of the celiac intestinal antibody (CIA) pattern through eight antibody assessments in fecal supernatants from patients with celiac disease.

摘要: Abstract Background Detection of anti-transglutaminase, anti-endomysium and anti-gliadin antibodies is commonly used to screen celiac disease patients. Besides that in serum, these are detectable culture supernatants oral, duodenal colonic biopsy samples, saliva, gut lavage fluid fecal supernatants. Our aim was extend the intestinal antibody pattern from patients with disease. Methods The obtained 25 12 healthy volunteers were determine IgA IgG1 by immunofluorescence analysis, IgG anti-deamidated gliadin peptides, IgA/IgG anti-transglutaminase/deamidated peptides anti-actin enzyme-linked immunosorbent assay. Results found 11 (44.0%) none ( p  = 0.0066). levels determined significantly higher  = 0.0005,  = 0.0018,  = 0.0061  = 0.0477, respectively) than those measured volunteers. ROC curve analysis showed a diagnostic significance anti-transglutaminase (AUC = 0.862,  = 0.0003) tests. Conclusions data supernatants, thus increasing knowledge humoral immunity Further studies needed better evaluate role tests identifying