Effects of thymoquinone on isolated and cellular proteasomes.
作者： Valentina Cecarini , Luana Quassinti , Alessia Di Blasio , Laura Bonfili , Massimo Bramucci
DOI: 10.1111/J.1742-4658.2010.07629.X
关键词:
摘要: Thymoquinone, a naturally derived agent, has been shown to possess antioxidant, antiproliferative and proapoptotic activities. In the present study, we explored thymoquinone effects on proteasomal complex, major system involved in removal of damaged, oxidized misfolded proteins. purified 20S complexes, subunit-dependent composition-dependent inhibition was observed, chymotrypsin-like trypsin-like activities were most susceptible treatment. U87 MG T98G malignant glioma cells treated with thymoquinone, 26S proteasome activity measured. Inhibition complex evident both cell lines, but predominantly cells, accompanied by accumulation ubiquitin conjugates. Accumulation p53 Bax, two substrates activity, observed lines. Our results demonstrate that induces selective time-dependent inhibition, isolated enzymes glioblastoma suggest this mechanism could be implicated induction apoptosis cancer cells.
core.ac.uk
cabdirect.org
unicam.it
sci-hub.se 参考文章(53)
Laura Bonfili, Valentina Cecarini, Manila Amici, Massimiliano Cuccioloni, Mauro Angeletti, Jeffrey N. Keller, Anna M. Eleuteri, Natural polyphenols as proteasome modulators and their role as anti-cancer compounds FEBS Journal. ,vol. 275, pp. 5512- 5526 ,(2008) , 10.1111/J.1742-4658.2008.06696.X
EG Meir Van, T Kikuchi, M Tada, H Li, AC Diserens, BE Wojcik, HJ Huang, T Friedmann, WK Cavenee, None, Analysis of the p53 Gene and Its Expression in Human Glioblastoma Cells Cancer Research. ,vol. 54, pp. 649- 652 ,(1994)
Josianne Al-Hmaira, Hala Gali-Muhtasib, Roland Hartig, Albert Roessner, Mona Diab-Assaf, Carsten Boltze, Regine Schneider-Stock, Thymoquinone extracted from black seed triggers apoptotic cell death in human colorectal cancer cells via a p53-dependent mechanism. International Journal of Oncology. ,vol. 25, pp. 857- 866 ,(2004) , 10.3892/IJO.25.4.857
P M Gallop, R Flückiger, M A Paz, H M Kagan, A Boak, Specific detection of quinoproteins by redox-cycling staining. Journal of Biological Chemistry. ,vol. 266, pp. 689- 692 ,(1991) , 10.1016/S0021-9258(17)35225-0
Bert Vogelstein, David Lane, Arnold J. Levine, Surfing the p53 network Nature. ,vol. 408, pp. 307- 310 ,(2000) , 10.1038/35042675
P A Crooks, O A Ghosheh, D R Worthen, The in vitro anti-tumor activity of some crude and purified components of blackseed, Nigella sativa L. Anticancer Research. ,vol. 18, pp. 1527- 1532 ,(1998)
C. Marchini, M. Angeletti, A.M. Eleuteri, A. Fedeli, E. Fioretti, Aspirin modulates LPS-induced nitric oxide release in rat glial cells Neuroscience Letters. ,vol. 381, pp. 86- 91 ,(2005) , 10.1016/J.NEULET.2005.02.002
Hala U Gali-Muhtasib, Wassim G Abou Kheir, Lynn A Kheir, Nadine Darwiche, Peter A Crooks, None, Molecular pathway for thymoquinone-induced cell-cycle arrest and apoptosis in neoplastic keratinocytes. Anti-Cancer Drugs. ,vol. 15, pp. 389- 399 ,(2004) , 10.1097/00001813-200404000-00012
Ahmed Shoieb, Mona Elgayyar, Paul Dudrick, John Bell, Patricia Tithof, In vitro inhibition of growth and induction of apoptosis in cancer cell lines by thymoquinone International Journal of Oncology. ,vol. 22, pp. 107- 113 ,(2003) , 10.3892/IJO.22.1.107
Panagiotis J. Vlachostergios, Anna Patrikidou, Danai D. Daliani, Christos N. Papandreou, The ubiquitin-proteasome system in cancer, a major player in DNA repair. Part 1: post-translational regulation Journal of Cellular and Molecular Medicine. ,vol. 13, pp. 3006- 3018 ,(2009) , 10.1111/J.1582-4934.2009.00824.X