The prognostic potential and oncogenic effects of PRR11 expression in hilar cholangiocarcinoma

摘要: // Ying Chen 1,* , Zhanshan Cha 2,* Wenzheng Fang 3,* Baohua Qian 2 Wenlong Yu 4 Wenfeng Li 5 Guanzhen 6,7 and Yong Gao 7 1 Department of Pathology, Changhai Hospital, Shanghai, China Transfusion, 3 Oncology, Fuzhou General Fuzhou, Fujian Province, Surgery, Eastern Hepatobiliary Radiation First Affiliated Hospital Wenzhou Medical College, Wenzhou, Zhejiang, 6 Changzheng East Tongji University School Medicine, * These authors have contributed equally to this work Correspondence to: Yu, email: Li, Keywords : PRR11; hilar cholangiocarcinoma; oncogene; prognosis Received December 29, 2014 Accepted April 10, 2015 Published May 04, Abstract PRR11 is a newly identified oncogene in lung cancer, yet its role others tumors remains unclear. Gastrointestinal tissue microarrays were used evaluate expression association with clinical outcome was analyzed patients cholangiocarcinoma. Overexpression observed esophageal, gastric, pancreatic, colorectal, Expression correlated lymph node metastasis CA199 level two HC patient cohorts. After an R0 resection, high found be independent indicator recurrence ( P = 0.001). In cell culture, silencing resulted decreased cellular proliferation, migration, tumor growth QBC939 cells. Microarray analysis revealed that several genes involved adhesion, migration altered PRR11-knockout cells, including: vimentin (VIM), Ubiquitin carboxyl-terminal hydrolase (UCHL1), early response protein (EGR1), System A amino acid transporter1 (SNAT1). Silencing inhibited the UCHL1, EGR1, SNAT1 proteins, immunoassays revealing significant correlation among levels these four proteins. results indicate prognostic for HC.