S100A14, a member of the EF-hand calcium-binding proteins, is overexpressed in breast cancer and acts as a modulator of HER2 signaling.

摘要: HER2 is overexpressed in 20–25% of breast cancers. Overexpression an adverse prognostic factor and correlates with decreased patient survival. stimulates tumorigenesis via a number intracellular signaling molecules, including PI3K/AKT MAPK/ERK. S100A14, one member the S100 protein family, significantly associated outcome cancer patients. Here, for first time, we show that S100A14 are coexpressed invasive specimens, there significant correlation between expression levels two proteins by immunohistochemistry. colocalized plasma membrane tissue cells cell lines BT474 SK-BR3. We demonstrate binds directly to co-immunoprecipitation pull-down assays. Further study shows residues 956–1154 domain residue 83 essential binding. Moreover, observe decrease phosphorylation, downstream signaling, HER2-stimulated proliferation S100A14-silenced MCF-7, BT474, SK-BR3 cells. Our findings suggest functions as modulator provide mechanistic evidence its role progression.