A nucleotide-specific polymerase chain reaction assay to differentiate rabies virus biotypes in South Africa.

摘要: NEL, L.H., BINGHAM, J. JACOBS, J.A. & JAFTHA, J.B. 1998. A nucleotide-specific polymerase chain reaction assay to differentiate rabies virus biotypes in South Africa. Onderstepoort Journal of Veteri­ nary Research, 65:297-303 Antigenic and nucleotide sequence analyses have shown that two distinct are circulating One these typically infects members the family Canidae, while other comprises a heterogeneous group apparently indigenous viruses, infecting Viverridae family. In recent times, it has become evident considerable amount cross­ infection may occur manifestation viverrid non-viverrid animals particular ap­ pears more commonplace. Consequently, need rapidly distinguish between increasingly important efforts monitor epidemiology southern African region. this study, nested (PCR) was developed groups viruses. Consensus oligonucleotides were used amplify cytoplasmic domain glycoprotein adjacent inter­ genic region . The resultant amplicon subsequently as template second round hemi­ PCR presence type-specific primers, thereby successfully generating amplicons characteristic size for each biotype.