Differentiation of human embryonic stem cells to cardiomyocytes by coculture with endoderm in serum-free medium.

摘要: Many of the applications envisaged for human embryonic stem cells (hESC) undergoing cardiomyogenesis require that differentiation procedure is robust and high yield. For many hESC lines currently available this a challenge; beating areas are often obtained but subsequent analysis shows only few (<1%) cardiomyocytes actually present. Here authors provide protocol based on serum-free coculture with mouse endoderm-like cell line (END2), which yields cultures containing average 25% two widely lines, hES2 hES3. The also variant growth aggregates/embryoid bodies in END2-conditioned medium method dissociating aggregates without compromising cardiomyocyte viability so they can be used transplantation into animals or further (electrophysiological) analysis.