Modulation of human cytochrome P450 3A4 (CYP3A4) and P-glycoprotein (P-gp) in Caco-2 cell monolayers by selected commercial-source milk thistle and goldenseal products.
作者： Jason W. Budzinski , Vance L. Trudeau , Cathy E. Drouin , Mitra Panahi , J. Thor Arnason
DOI: 10.1139/Y07-091
关键词:
摘要: In this study, we used an in vitro Caco-2 cell monolayer model to evaluate aqueous extracts of commercial-source goldenseal (Hydrastis canadensis) and milk thistle (Silybum marianum) capsule formulations, their marker phytochemicals (berberine silibinin, respectively), as well dillapiol, vinblastine, the HIV protease inhibitor saquinavir for ability modulate CYP3A4 ABCB1 expression after short-term exposure (48 h). Both upregulation downregulation was observed with varying concentrations two natural health products (NHPs). highly responsive our system, showing a strong dose-dependent modulation by dillapiol (upregulation) flavonolignan silibinin (downregulation). largely unresponsive cellular appears be little value biomarker under experimental conditions. Therefore, gene can serve important assessment NHP-drug interactions.
nrcresearchpress.com参考文章(36)
Mackey K, Chomczynski P, Short technical reports. Modification of the TRI reagent procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources. BioTechniques. ,vol. 19, pp. 942- 945 ,(1995)
Anthony Krantis, John T. Arnason, Jason W. Budzinski, Meredith S. Foster, Keith D. Gallicano, Brian C. Foster, Shurjeel Choudri, Susan Vandenhoek, An in vitro evaluation of human cytochrome P450 3A4 and P-glycoprotein inhibition by garlic. Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences. ,vol. 4, pp. 176- ,(2001)
Jeffrey Barry Harborne, Herbert Baxter, Gerard Peter Moss, Phytochemical dictionary. A handbook of bioactive compounds from plants. Published in <b>1999</b> in London by Taylor and Francis. ,(1999)
Dolly A Parasrampuria, Marianne V Lantz, Leslie Z Benet, A Human Lymphocyte Based Ex Vivo Assay to Study the Effect of Drugs on P-glycoprotein (p-Gp) Function1 Pharmaceutical Research. ,vol. 18, pp. 39- 44 ,(2001) , 10.1023/A:1011070509191
Carolyn L. Cummins, Lara M. Mangravite, Leslie Z. Benet, Characterizing the expression of CYP3A4 and efflux transporters (P-gp, MRP1, and MRP2) in CYP3A4-transfected Caco-2 cells after induction with sodium butyrate and the phorbol ester 12-O-tetradecanoylphorbol-13-acetate. Pharmaceutical Research. ,vol. 18, pp. 1102- 1109 ,(2001) , 10.1023/A:1010914624111
B GURLEY, S GARDNER, M HUBBARD, D WILLIAMS, W GENTRY, J CARRIER, I KHAN, D EDWARDS, A SHAH, In vivo assessment of botanical supplementation on human cytochrome P450 phenotypes: Citrus aurantium, Echinacea purpurea, milk thistle, and saw palmetto Clinical Pharmacology & Therapeutics. ,vol. 76, pp. 428- 440 ,(2004) , 10.1016/J.CLPT.2004.07.007
Christoph Wandel, Alastair J. J. Wood, Richard B. Kim, F. Peter Guengerich, Grant R. Wilkinson, Shama Kajiji, P-glycoprotein and cytochrome P-450 3A inhibition: dissociation of inhibitory potencies. Cancer Research. ,vol. 59, pp. 3944- 3948 ,(1999)
B GURLEY, S GARDNER, M HUBBARD, D WILLIAMS, W GENTRY, I KHAN, A SHAH, In Vivo Effects of Goldenseal, Kava Kava, Black Cohosh, and Valerian on Human Cytochrome P450 1A2, 2D6, 2E1, and 3A4/5 Phenotypes Clinical Pharmacology & Therapeutics. ,vol. 77, pp. 415- 426 ,(2005) , 10.1016/J.CLPT.2005.01.009
Raman Venkataramanan, Bernard Komoroski, Stephen Strom, In vitro and in vivo assessment of herb drug interactions. Life Sciences. ,vol. 78, pp. 2105- 2115 ,(2006) , 10.1016/J.LFS.2005.12.021
Barbara Ameer, Randy A. Weintraub, Drug interactions with grapefruit juice Clinical Pharmacokinectics. ,vol. 33, pp. 103- 121 ,(1997) , 10.2165/00003088-199733020-00003