Liposomes coated with chemically modified dextran interact with human endothelial cells.

摘要: Some liposomal formulations are now in clinical use. New applications biology and medicine using targeted liposomes remain an intensive research area. In this context, constituted of phosphatidylcholine (PC), phosphatidylethanolamine (PE), cholesterol (70/10/20 mol %) were prepared by detergent dialysis coated with dextran (Dx) or functionalized (FDx), both hydrophobized a anchor which penetrates the lipid bilayer during vesicle formation. The coating these polysaccharides was performed because chemically modified but not native Dx interacted vascular cells. liposome uptake human endothelial cells followed uncoated radiolabeled neutral (3H-cholesterol) polar phospholipid (14C-PC). results indicated for radiolabels preferential FDx-coated compared to Dx-coated liposomes. Addition culture medium calcium up 10 mM further enhanced level rate incorporation liposomes, whereas interaction poorly affected. Liposome membranes then labeled N-(lissamine rhodamine B sulfonyl)diacyl-PE observed fluorescence microscopy. punctate intracellular incubated at 37°C fluorolabeled is indicative localization within endocytotic pathway Altogether, data demonstrate that FDx enable specific interactions culture. Consequently, bioactive polymers represent attractive approach as materials use drug delivery vehicles targeting © 1999 John Wiley & Sons, Inc. J Biomed Mater Res, 44, 140–148, 1999.