Immunomodulatory Effects of Polysaccharide from Marine Fungus Phoma herbarum YS4108 on T Cells and Dendritic Cells
作者： Song Chen , Ran Ding , Yan Zhou , Xian Zhang , Rui Zhu
DOI: 10.1155/2014/738631
关键词:
摘要: YCP, as a kind of natural polysaccharides from the mycelium marine filamentous fungus Phoma herbarum YS4108, has great antitumor potential via enhancement host immune response, but little is known about molecular mechanisms. In present study, we mainly focused on effects and mechanisms YCP specific immunity mediated by dendritic cells (DCs) T cells. cell /DC activation-related factors including interferon- (IFN-) γ, interleukin-12 (IL-12), IL-4 were examined with ELISA. Receptor knock-out mice fluorescence-activated sorting are used to analyze YCP-binding receptor DCs. RT-PCR utilized measure MAGE-A3 for analyzing tumor-specific killing effect. our demonstrated can provide second signal activation, proliferation, IFN-γ production through binding toll-like receptor- (TLR-) 2 TLR-4. could effectively promote IL-12 secretion expression markers (CD80, CD86, MHC II) TLR-4 Antigen-specific against mouse melanoma was strengthened activation capacity DCs YCP. The data supported that exhibit immunomodulatory
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